Growth of Spores Gracilaria sp. Become A Planlet Using Provasoli's Enrich Seawater Media
Keywords:
Cystocarp, Gracilaria sp, PES, Plantlets, SporeAbstract
This research aims to determine the production technique for Gracilaria sp seaweed plantlets produced from spores, conducted in August - November 2024 at the Fish Reproduction and Genetics Laboratory, Pangkep State Polytechnic of Agriculture. Parameters that influenced spore growth were observed including light intensity, temperature and salinity of the culture medium. The research began by taking the thallus of Gracilaria sp. containing cystocarp and it was cut 1.5-2 cm long and soaked in sterile sea water with a salinity of 30 ppt. The thallus were maintained in a petri dish containing a culture medium PES (Provasoli's Enrich Seawater) fertilizer at a dose of 5 mL PES in 1000 mL seawater. Observation of the release of spores from the cystocarp was carried out 24 hours after planting. Observation of the growth of spores into plantlets was carried out at room temperature 26-27℃, using a microscope with 10-20X magnification. The results were that spore release occurred at DOC-2 and spore attachment occurred at DOC-9. The spore size increases up to DOC-14. Spore buds began to appear at DOC-18 and spore buds began to grow at DOC-22. The increase in the length of the spore shoots began at DOC-32 and the shoots began to produce branches at DOC-40. In DOC-52 the spore shoots have become plantlets. This research indicates that PES fertilizer can be a good medium for the growth of Gracilaria sp spores to become plantlets at a light intensity of 12 hours of light and 12 hours of darkness.
References
Asni, A. (2015). Analysis of seaweed production (Kappaphycus alvarezii) based on season and distance of cultivation location in bantaeng regency waters. Indonesian Aquatics Journal, 6 (2), 140–153.
Asrul, I., Hamzah, H., & Salam, NI (2020). The effect of commercial npk fertilizer application on the release of seaweed spores Gracilaria sp. Octopus: Journal of Fisheries Science, 9 (1), 19–24.
Dworjanyn, S. (2020). Utilization of spores attached to rope as a seed source of Gracilaria Seaweeds (Gracilaria sp.). 36. Https://www.researchgate.net/profile/akmal-akmal-5/publication/361669508_utilization_of_spore_attached_to_rope_as_a_seed_source_of_gracilaria_seaweeds_gracilaria_sp_utilization_of_spore_attached_to_rope_as_seed_source_of_gracilaria_seaweed_grac
Erlania, E., & Radiarta, IN (2014). Management of sustainable seaweed (Kappaphycus alvarezii) Aquaculture in the context of climate change mitigation. Indonesian Aquaculture Journal, 9 (1), 65–72.
Hesti Mulyaningrum, SR, Parenrengi, A., & Suryati, E. (2015). Growth and development of seaweed explants Gracilaria verrucosa and Gracilaria gigas during acclimatization in ponds. Ilmu Kelautan: Indonesian Journal of Marine Sciences, 20 (3).
Hirijal, M. (2024). Pegaruh lama penyinaran terhadap pelepasan dan penempelan spora.SKRIPSI. Jurusan Budidaya Perikanan. Politeknik Pertanian Negeri Pangkajene Kepulauan.
Lideman, Elman, A., Hartanto, N., Agusanty, H., Said, AI, & Jamal, K. (2016). Produksi bibit Gracilaria laut (Gracilaria sp.) melalui kultur spora pada tali. Petunjuk Teknis. Takalar Brackish Water Aquaculture Center.
Lideman, G. N. N., Noro, T., & Terada, R. (2013). Effect of temperature and light on the photosynthesis as and Kappaphycus sp (Strain Sumba) from Indonesia. Journal of Applied Phycology, 25 (25).
Nadhif, H. (2021). The Effect of acetobacter aceti concentration and fermentation time on acetic acid levels of Gracilaria sp. Seaweed UIN Ar-Raniry.
Safia, W. (2020). Nutritional and bioactive content of seaweed (Euchema cottonii) with hanging raft method at different depths. Indonesian Journal of Fisheries Product Processing, 23 (2), 261–271.
Sipahutar, YH, Ma'roef, AFF, Febrianti, AA, Nur, C., Savitri, N., & Utami, SP (2021). Characteristics of tilapia fish sausage (Oreochromis niloticus) with the addition of seaweed flour (Gracilaria sp.). Journal of Fisheries and Marine Extension, 15 (1), 69–84.
